Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/12/17"

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Original DTT concentration 1M
 
Original DTT concentration 1M
1M(x) = (100x10-3M)(1mL)
+
 
 +
1M(x) = (100x10<sup>-3</sup>M)(1mL)
 +
 
 
x = 0.1mL = 100μL in 900μL 4% TEA
 
x = 0.1mL = 100μL in 900μL 4% TEA
 +
 
4%(900μL) = 36μL TEA in 864μL PBS
 
4%(900μL) = 36μL TEA in 864μL PBS
 +
 
OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS
 
OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS
  

Revision as of 08:51, 17 December 2013

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1) Prepare 100mM DTT in 4% TEA

From 5/29:

Original DTT concentration 1M

1M(x) = (100x10-3M)(1mL)

x = 0.1mL = 100μL in 900μL 4% TEA

4%(900μL) = 36μL TEA in 864μL PBS

OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS