Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/12/16"

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(NEW Telomeric DNA)
(DNA Attachment)
Line 26: Line 26:
 
==DNA Attachment==
 
==DNA Attachment==
  
attach DNA to AuNP
+
original [AuNP] = 23.5nM
 +
 
 +
original [DNA] 63.1uM
 +
 
 +
attach DNA to AuNP in 75:1 ratio
 +
 
 +
 
  
  

Revision as of 10:10, 16 December 2013

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NEW Telomeric DNA

thiol-8 repeat GTTAGGG

Tm=69.2°C

MW=19,007.5

38.2OD= 62.9nm = 1.20mg

1.20mg x mol/19,007.5g x 1g/1000mg = 6.31x10-8mol

dilute in 1mL 1X PBS

6.31x10-8mol / 1mL x 1000mL/1L = 6.31x10-5M

6.31x10-5M x 106μM/1M = 63.1μM

DNA Attachment

original [AuNP] = 23.5nM

original [DNA] 63.1uM

attach DNA to AuNP in 75:1 ratio