User:Allison K. Alix/Notebook/Thesis Research/2013/11/05
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Calculating AuNP/DNA Concentration
AuNP: abs @ 532 = 0.03
ε = 92,382,878
c = 3.2473x10-10M = 0.325nM
DNA: abs @ 254 = 0.399
ε = 607,500
c = 6.5679E-07M = 656.6nM
Addition of MB
1) Add 6uL 7.61uM MB to 50uL AuNP/DNA Solution
2) Heat for 25 min at ~76.0°C
3) Run FCS Measurements. Begin with pure sample. Because the AuNP concentration is so low (290pM) it might be hard to get signal from anything lower.
The UV-Vis spectra from the beacon alone did not yield the expected results. Not only were the spectra out of order (expecting to see highest concentration have the highest absorbance) but it was jagged, and not smooth like ti normally is. This might be attributed to the fact that the samples were not freshly made (prepared 10/30) and the DNA could have either clumped together causing inaccurate scattering patterns of light or settled to the bottom.