Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/11/05"

From OpenWetWare
Jump to: navigation, search
(Calculating AuNP/DNA Concentration)
(Addition of MB)
Line 34: Line 34:
==Addition of MB==
==Addition of MB==
1) Add 6uL 7.61uM MB to 50uL AuNP/DNA Solutio

Revision as of 10:14, 5 November 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

To Do

  • Run UV-Vis of MB samples to determine concentraiton
  • Add MB to AuNP/DNA Sample. Run FCS.


OWW AUNP DNA pic.png

Calculating AuNP/DNA Concentration

AuNP: abs @ 532 = 0.03

ε = 92,382,878

c= A/εb

c = 3.2473x10-10M = 0.325nM

DNA: abs @ 254 = 0.399

ε = 607,500

c = 6.5679E-07M = 656.6nM

Addition of MB

1) Add 6uL 7.61uM MB to 50uL AuNP/DNA Solutio