User:Allison K. Alix/Notebook/Thesis Research/2013/10/30

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png October 30th, 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

To Do

  • Prepare MB samples and obtain UV-Vis Spectra. Calculate the concentration of each.
  • Attach DNA to AuNP (no silica coating) obtain concentration of DNA attached to AuNP, attach MB.

Sample Preparation

Prepare 150uL of the following MB samples with the following concentrations:

22.4nM, 17.9nM, 14.9nM, 12.8nM, 11.2nM, 9.9nM, 8.9nM, and 8.2nM

Original MB concentration: 7.61uM

Dilute to 895nM

7.61x10-6M (x) = 895x10-9M(75uL)

x = 8.8uL in 66.2uL PBS