User:Allison K. Alix/Notebook/Thesis Research/2013/10/09: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> October 9th, 2013</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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[DNA] = 875nM | [DNA] = 875nM | ||
==Dilutions for DNA:MB samples== | |||
1) | |||
{| class="wikitable" | |||
|- | |||
! Dilution Factor !! [Au@SiO<sub>2</sub>] !! [DNA] !! [MB] | |||
|- | |||
| None (stock) || 3.06nM || 404nM || 3182nM | |||
|- | |||
| 5X || 612pM || 80.8nM || 636.4nM | |||
|- | |||
| 10X || 306pM || 40.4nM || 318.2M | |||
|- | |||
| 100X || 30.6pM || 4.04nM || 31.82nM | |||
|- | |||
| 1000X || 3.06pM || 404pM || 3.182nM | |||
|- | |||
| 10000X || 306fM || 40.4pM || 318.2pM | |||
|} | |||
2) | |||
{| class="wikitable" | |||
|- | |||
! Dilution Factor !! [Au@SiO<sub>2</sub>] !! [DNA] !! [MB] | |||
|- | |||
| None (stock) || 4.88nM || 803nM || 887nM | |||
|- | |||
| 5X || 975pM || 160.6nM || 177.4nM | |||
|- | |||
| 10X || 488pM || 80.3nM || 88.7M | |||
|- | |||
| 100X || 48.8pM || 8.03nM || 8.87nM | |||
|- | |||
| 1000X || 4.88pM || 803pM || 887pM | |||
|- | |||
| 10000X || 488fM || 80.3pM || 88.7pM | |||
|} | |||
3) | |||
{| class="wikitable" | |||
|- | |||
! Dilution Factor !! [Au@SiO<sub>2</sub>] !! [DNA] !! [MB] | |||
|- | |||
| None (stock) || 5.31nM || 875nM || 292.7nM | |||
|- | |||
| 5X || 1.062nM || 175nM || 58.54nM | |||
|- | |||
| 10X || 531pM || 87.5nM || 29.3M | |||
|- | |||
| 100X || 53.1pM || 8.75nM || 2.93nM | |||
|- | |||
| 1000X || 5.31pM || 875pM || 293pM | |||
|- | |||
| 10000X || 531fM || 87.5pM || 29.3pM | |||
|} | |||
==Calculations for preparing diltuions== | |||
* Need 20uL for each dilution | |||
{| class="wikitable" | |||
|- | |||
! Dilution Factor !! Solution to make dilution from !! Volume of solution !! Volume of PBS | |||
|- | |||
| 5X || stock || 4uL || 16uL | |||
|- | |||
| 10X || stock || 3uL || 27uL | |||
|- | |||
| 100X || 10X || 3uL || 27uL | |||
|- | |||
| 1000X || 100X || 3uL || 27uL | |||
|- | |||
| 10000X || 1000X || 3uL || 27uL | |||
|} | |||
Revision as of 13:14, 5 November 2013
October 9th, 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
To Do
Preparing new Au@SiO2-DNA/MB solutions
1) 1:3 DNA:MB Original [DNA] = 910nM Original [MB] = 7610nM MB should be 2730nM
NEW CONCENTRATIONS: MB = 3.182uM DNA = 404uM (This is actually a 1:8 ratio....) 2) 1:1 Added 3.3uL 7.61uM MB to 25uL Au@Si/DNA solution [MB] = 887nM [DNA] = 803nM 3) 3:1 DNA:MB Added 1uL MB to 25uL Au@Si/DNA solution [MB] = 292.7nM [DNA] = 875nM Dilutions for DNA:MB samples1)
2)
3)
Calculations for preparing diltuions
|