User:Allison K. Alix/Notebook/Thesis Research/2013/10/08

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Revision as of 10:33, 8 October 2013 by Allison K. Alix (talk | contribs) (Calculating ω0 using 10000X diluted green fluorescent beads)
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Calculations

τD = (ω023ηπd)/4kT

where τD is the diffusion time of the sample

ω0 is the radius of the spot

η is the viscosity of the solvent (water), 0.001028kg/m s

d is the diameter of the particle

k is Boltzmann's constant = 1.3806488 × 10-23 m2 kg s-2-2 K-1

T is the temperature of the room (18.7 °C, 291.7K)

Calculating ω0 using 10000X diluted green fluorescent beads

average τD = 36.3137ms = 0.0363137 sec (taken from 10000X beads on 9/4, 9/11, and 9/12)

sqr rt [(0.0363137s)(4)(1.3806488 × 10-23 m2 kg s-2 K-1)(291.7K)]/[3(0.001028kg/m s)(pi)(2x10-7) = 5.49x10-7 m

ω0 = 0.549um

  • diameter was calculated for all samples with an average of 17.8 nm (not including the diameter calculated from the 61.33pM sample)

FCS measurements of MB only

prepare the MB at the following concentrations: