Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/10/08"

From OpenWetWare
Jump to: navigation, search
(Calculations)
(5 intermediate revisions by the same user not shown)
Line 1: Line 1:
 
{|{{table}} width="800"
 
{|{{table}} width="800"
 
|-
 
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
+
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> October 8th, 2013</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|-
 
|-
Line 22: Line 22:
 
T is the temperature of the room (18.7 °C, 291.7K)
 
T is the temperature of the room (18.7 °C, 291.7K)
  
==Calculating ω<sub>0</sub>== using 10000X diluted green fluorescent beads==
+
==Calculating ω<sub>0</sub> using 10000X diluted green fluorescent beads==
  
 
average τ<sub>D</sub> = 36.3137ms = 0.0363137 sec (taken from 10000X beads on 9/4, 9/11, and 9/12)
 
average τ<sub>D</sub> = 36.3137ms = 0.0363137 sec (taken from 10000X beads on 9/4, 9/11, and 9/12)
  
sqr rt [(0.0363137s)(4)(1.3806488 × 10-23 m<sup>2</sup> kg s<sup>-2</sup>-2 K<sup>-1</sup>)(291.7)]/[3(0.001028kg/m s)(pi)(2x10<sup>-7</sup>) =
+
sqr rt [(0.0363137s)(4)(1.3806488 × 10-23 m<sup>2</sup> kg s<sup>-2</sup> K<sup>-1</sup>)(291.7K)]/[3(0.001028kg/m s)(pi)(2x10<sup>-7</sup>) = 5.49x10<sup>-7</sup> m
 +
 
 +
'''ω<sub>0</sub> = 0.549um'''
 +
 
 +
* diameter was calculated for all samples with an average of 17.8 nm (not including the diameter calculated from the 61.33pM sample)
 +
 
 +
==FCS measurements of MB only==
 +
 
 +
prepare the MB at the following concentrations:
 +
 
 +
 
  
  

Revision as of 12:17, 5 November 2013

Owwnotebook icon.png October 8th, 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Calculations

τD = (ω023ηπd)/4kT

where τD is the diffusion time of the sample

ω0 is the radius of the spot

η is the viscosity of the solvent (water), 0.001028kg/m s

d is the diameter of the particle

k is Boltzmann's constant = 1.3806488 × 10-23 m2 kg s-2-2 K-1

T is the temperature of the room (18.7 °C, 291.7K)

Calculating ω0 using 10000X diluted green fluorescent beads

average τD = 36.3137ms = 0.0363137 sec (taken from 10000X beads on 9/4, 9/11, and 9/12)

sqr rt [(0.0363137s)(4)(1.3806488 × 10-23 m2 kg s-2 K-1)(291.7K)]/[3(0.001028kg/m s)(pi)(2x10-7) = 5.49x10-7 m

ω0 = 0.549um

  • diameter was calculated for all samples with an average of 17.8 nm (not including the diameter calculated from the 61.33pM sample)

FCS measurements of MB only

prepare the MB at the following concentrations: