Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/09/24"

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==Entry title==
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==Calculating [AuNP] in Au@SiO2/DNA-MB solutions==
* Insert content here...
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*Original [Au@SiO2] = 5nM before attaching DNA
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1) Addition of 5uL APS
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(1mL)(5nM)=(1.005mL)(x)
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x = 4.975nM
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2) Addition of 1.5mL Sulfo-SMCC
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(1.005mL)(4.975nM) = (x)(2.505mL)
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x = 1.996nM
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3) Centrifugation, removal of supernatant, and redispersion in 530uL KP buffer
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(1.996nM)(2.505mL) = x(0.530mL)
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x = 9.43nM
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4) Addition of 270uL DNA
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(9.43nM)(530uL)=x(800uL)
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x = 6.25nM
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Revision as of 06:18, 24 September 2013

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Calculating [AuNP] in Au@SiO2/DNA-MB solutions

  • Original [Au@SiO2] = 5nM before attaching DNA

1) Addition of 5uL APS

(1mL)(5nM)=(1.005mL)(x)

x = 4.975nM

2) Addition of 1.5mL Sulfo-SMCC

(1.005mL)(4.975nM) = (x)(2.505mL)

x = 1.996nM

3) Centrifugation, removal of supernatant, and redispersion in 530uL KP buffer

(1.996nM)(2.505mL) = x(0.530mL)

x = 9.43nM

4) Addition of 270uL DNA

(9.43nM)(530uL)=x(800uL)

x = 6.25nM