Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/09/17"

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(FCS Curves)
(FCS Curves)
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[[Image:1pM_Antibody-Alexa_532_pic.png]]
 
[[Image:1pM_Antibody-Alexa_532_pic.png]]
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==Attaching MB to Au@SiO2-DNA==
 +
 +
1) Add 13.3 uL 7.61uM MB to Au@SiO2-DNA
 +
 +
2) Hybridize by heating for 20 min @ 75C
 +
 +
3) Slowly cool to room temperature.
  
  

Revision as of 09:28, 17 September 2013

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To Do

  • Run Antibody-Alexa 532 Samples
  • Run AuNp@SiO2/DNA_MB Samples

FCS Curves

10nM Antibody-Alexa 532 pic.png

1nM Antibody-Alexa 532 pic.png

100pM Antibody-Alexa 532 pic.png

10pM Antibody-Alexa 532 pic2.png

1pM Antibody-Alexa 532 pic.png

Attaching MB to Au@SiO2-DNA

1) Add 13.3 uL 7.61uM MB to Au@SiO2-DNA

2) Hybridize by heating for 20 min @ 75C

3) Slowly cool to room temperature.