Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/09/12"

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(FCS Measurements of Antibody-Alexa 532 Pt 2)
(FCS Measurements of Antibody-Alexa 532 Pt 2)
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| 10pM || 9.60x10<sup>2</sup> ||  
 
| 10pM || 9.60x10<sup>2</sup> ||  
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| 1pM || 1.79x10<sup>3</sup> ||
 
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Revision as of 10:24, 12 September 2013

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FCS Measurements of Antibody-Alexa 532 Pt 2

  • Green fluorescent beads were first used to calibrate the FCS (See spectra below)
  • The count rate of each Antibody/Alexa 532 sample was monitored
Sample Concentration TDC counts/sec
100nM 1.90x104
10nM 3.50x103
1nM 2.30x103
100pM 1.98x103
10pM 9.60x102
1pM 1.79x103

FCS Spectra

1000X diluted green beads pic.png