User:Allison K. Alix/Notebook/Thesis Research/2013/07/25

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

To Do

  • Repeat Silica-Coating procedure using water to dilute nanoparticles instead of PBS. PBS is likely the cause of the aggregation we have been observing.[1]
  • Repeat ThT-DNA fluorescence procedure to show that fluorescence increases with increasing number of G-quadruplexes formed.

Silica-Coating Part 1

1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*)

2)

ThT/DNA attachment

1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)

a) 100nM

b) 300nM

c) 500nM

d) 700nM

e) 900nM

      • Original Concentrations of 4 and 8-repeats are 265.6uM and 142.8uM, respectively***

4-repeat

1uM(1mL)=(265.6uM)x

x = 3.77uL in 996.23uL

900nM--> 90uL 1uM in 10uL PBS

700nM--> 70uL in 30uL

500nM--> 50uL in 50uL

300nM--> 30uL in 70uL

100nM--> 10uL in 90uL





Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/25&oldid=712141"