Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/07/25"

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(ThT/DNA attachment)
(ThT/DNA attachment)
Line 36: Line 36:
 
'''4-repeat'''
 
'''4-repeat'''
  
x(500uL)=(265.6uM)(8.12)
+
x(500uL)=(265.6uM)(10)
  
x = 4.31uM
+
x = 5.31uM
  
1800nM-->  41.8uL in 58.2uL PBS
+
[100-([x(100)]/5.31)] =
  
1400nM--> 32.5uL in 67.5uL
+
1800nM--> 33.9uL in 58.2uL PBS add 7.9
  
1000nM--> 23.2uL in 76.8uL
+
1400nM--> 26.4uL in 67.5uL add 6.1
  
600nM--> 13.9uL in 86.1uL
+
1000nM--> 18.8uL in 76.8uL add 4.4
  
200nM--> 4.6uL in 95.4uL
+
600nM--> 11.3uL in 86.1uL add 2.6
 +
 
 +
200nM--> 3.8uL in 95.4uL add 0.8
  
 
'''8-repeat'''
 
'''8-repeat'''

Revision as of 08:01, 25 July 2013

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To Do

  • Repeat Silica-Coating procedure using water to dilute nanoparticles instead of PBS. PBS is likely the cause of the aggregation we have been observing.[1]
  • Repeat ThT-DNA fluorescence procedure to show that fluorescence increases with increasing number of G-quadruplexes formed.

Silica-Coating Part 1

1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*)

2)

ThT/DNA attachment

1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)

a) 100nM

b) 300nM

c) 500nM

d) 700nM

e) 900nM

NOTE: Original Concentrations of 4 and 8-repeats are 265.6uM and 142.8uM, respectively

4-repeat

x(500uL)=(265.6uM)(10)

x = 5.31uM

[100-([x(100)]/5.31)] =

1800nM--> 33.9uL in 58.2uL PBS add 7.9

1400nM--> 26.4uL in 67.5uL add 6.1

1000nM--> 18.8uL in 76.8uL add 4.4

600nM--> 11.3uL in 86.1uL add 2.6

200nM--> 3.8uL in 95.4uL add 0.8

8-repeat

142.8uM(10uL) = (x)(500uL)

x= 2.856uM

1800nM--> 63uL in 37uL PBS

1400nM--> 49uL in 51uL

1000nM--> 35uL in 65uL

600nM--> 21uL in 79uL

200nM--> 7uL in 93uL