Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/07/19"

From OpenWetWare
Jump to: navigation, search
(AuNP synthesis procedures)
(AuNP synthesis procedures)
Line 8: Line 8:
 
==AuNP synthesis procedures==
 
==AuNP synthesis procedures==
  
* We will perform two procedures and observe which produces the desired AuNP diameter (~10nm)
+
* We will perform three procedures and observe which produces the desired AuNP diameter (~10nm)
  
 
'''Procedure 1''' Taken from [[User:Allison K. Alix/Notebook/Thesis Research/2013/05/10]]
 
'''Procedure 1''' Taken from [[User:Allison K. Alix/Notebook/Thesis Research/2013/05/10]]
Line 19: Line 19:
  
 
4) Determine size and concentration of AuNP using UV-Vis
 
4) Determine size and concentration of AuNP using UV-Vis
 +
 +
Procedure 2[http://pubs.acs.org/doi/pdf/10.1021/la034348u]
 +
 +
1) Boil 50mL MilliQ water in a clean beaker
 +
 +
2) Add 0.5 mL of HAuCl4 solution (1% w/v)
 +
 +
3) Follow with 1.75 mL of sodium citrate solution (1% w/v)
 +
 +
4) Boil for about 10 minutes until solution color changes to bright red
 +
 +
5) Replace water lost to evaporation during boiling
 +
 +
6) If a higher concentration is desired, centrifuge at 14 000 rpm and resuspended in MilliQ water
 +
 +
Procedure 3[http://pubs.acs.org/doi/pdf/10.1021/ac0503198]
 +
 +
1) Bring 100 mL of solution containing 0.01 g of HAuCl4·3H2O to reflux
 +
 +
2) Add 3 mL of 1% sodium citrate solution while stirring
 +
 +
3) Boil solution for another 40 minutes
 +
 +
4) Cool to room temperature
  
  

Revision as of 06:47, 19 July 2013

Owwnotebook icon.png Synthesis of AuNP <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

AuNP synthesis procedures

  • We will perform three procedures and observe which produces the desired AuNP diameter (~10nm)

Procedure 1 Taken from User:Allison K. Alix/Notebook/Thesis Research/2013/05/10

1) Stir and heat to boiling 20mL of 1mM HAuCl4

2) Add 2mL 1% sodium citrate

3) Observe a purple to red color change

4) Determine size and concentration of AuNP using UV-Vis

Procedure 2[1]

1) Boil 50mL MilliQ water in a clean beaker

2) Add 0.5 mL of HAuCl4 solution (1% w/v)

3) Follow with 1.75 mL of sodium citrate solution (1% w/v)

4) Boil for about 10 minutes until solution color changes to bright red

5) Replace water lost to evaporation during boiling

6) If a higher concentration is desired, centrifuge at 14 000 rpm and resuspended in MilliQ water

Procedure 3[2]

1) Bring 100 mL of solution containing 0.01 g of HAuCl4·3H2O to reflux

2) Add 3 mL of 1% sodium citrate solution while stirring

3) Boil solution for another 40 minutes

4) Cool to room temperature