User:Allison K. Alix/Notebook/Thesis Research/2013/07/02

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Revision as of 06:24, 3 July 2013 by Allison K. Alix (talk | contribs) (Calibration Curves)
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FCS Measurements

Prepare the following oligio-D samples:

1) 150pM

2) 125pM

3) 100pM

Prepare the following DNA/MB samples

1) 100pM/80pM

2) 75pM/60pM

3) 50pM/40pM

  • Each sample was run 3 times for 500 seconds

FCS Data

Green Beads image OWW.png

The above image show an average correlation curve of 10,000x diluted green fluorescent beads

100pM OD OWW.png

125pM OD OWW.png

150pM OD OWW.png

50pM DNA OWW.png

75pM DNA OWW.png

100pM DNA OWW.png

Calibration Curves

DNA MB Cali Curve OWW.png

The above image is a calibration curve of 1/N vs. concentration for the DNA/MB samples. The 100pM and 75pM samples seem to follow the linear trend in relation to one another, however, the 50pM sample is a clear outlier. Because we did not see the pattern we are expecting for all three samples, we will prepare fresh solution tomorrow and run them again to see if there was some kind of error in sample preparation.