Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/07/02"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> FCS Measurements</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==FCS Data==
 
==FCS Data==
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[[Image:Green Beads image OWW.png]]
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The above image show an average correlation curve of 10,000x diluted green fluorescent beads
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[[Image:100pM OD OWW.png]]
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[[Image:125pM OD OWW.png]]
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[[Image:150pM OD OWW.png]]
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[[Image:50pM DNA OWW.png]]
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[[Image:75pM DNA OWW.png]]
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[[Image:100pM DNA OWW.png]]
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==Calibration Curves==
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[[Image:OD Cali Curve OWW.png]]
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The above image is a calibration curve of 1/N vs. concentration for the Oligo-D samples. We observed a very linear relationship between these samples, with an R<sup>2</sup> value of 0.99.
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[[Image:DNA MB Cali Curve OWW.png‎]]
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The above image is a calibration curve of 1/N vs. concentration for the DNA/MB samples. The 100pM and 75pM samples seem to follow the linear trend in relation to one another, however, the 50pM sample is a clear outlier. Because we did not see the pattern we are expecting for all three samples, we will prepare fresh solution tomorrow and run them again to see if there was some kind of error in sample preparation.
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Revision as of 11:36, 12 July 2013

Owwnotebook icon.png FCS Measurements <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

FCS Measurements

Prepare the following oligio-D samples:

1) 150pM

2) 125pM

3) 100pM

Prepare the following DNA/MB samples

1) 100pM/80pM

2) 75pM/60pM

3) 50pM/40pM

  • Each sample was run 3 times for 500 seconds

FCS Data

Green Beads image OWW.png

The above image show an average correlation curve of 10,000x diluted green fluorescent beads



100pM OD OWW.png

125pM OD OWW.png

150pM OD OWW.png



50pM DNA OWW.png

75pM DNA OWW.png

100pM DNA OWW.png

Calibration Curves

OD Cali Curve OWW.png

The above image is a calibration curve of 1/N vs. concentration for the Oligo-D samples. We observed a very linear relationship between these samples, with an R2 value of 0.99.

DNA MB Cali Curve OWW.png

The above image is a calibration curve of 1/N vs. concentration for the DNA/MB samples. The 100pM and 75pM samples seem to follow the linear trend in relation to one another, however, the 50pM sample is a clear outlier. Because we did not see the pattern we are expecting for all three samples, we will prepare fresh solution tomorrow and run them again to see if there was some kind of error in sample preparation.