Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/06/28"

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(FCS Sample Prep)
(Calculations)
Line 40: Line 40:
 
MOLECULAR BEACON:
 
MOLECULAR BEACON:
  
7.61μM (x) = 20nM (1000uL)
+
* 7.61μM (x) = 20nM (1000uL)
  
 
x = 2.62uL 7.61uM MB in 997.38uL PBS
 
x = 2.62uL 7.61uM MB in 997.38uL PBS
  
20nM (x) = (30uL)(10nM)
+
* 20nM (x) = (30uL)(10nM)
  
 
15uL in 15uL PBS
 
15uL in 15uL PBS
  
20nM (x) = 2nM(100uL)
+
* 20nM (x) = 2nM(100uL)
  
 
10uL in 90uL
 
10uL in 90uL
  
2nM (x) = 1nM (100uL)
+
* 2nM (x) = 1nM (100uL)
  
 
x = 50uL in 50uL PBS
 
x = 50uL in 50uL PBS
  
200pM (100uL) = x (1nM)
+
* 200pM (100uL) = x (1nM)
  
 
x = 20uL in 80uL PBS
 
x = 20uL in 80uL PBS
  
100pM(100uL) = (200pM)(x)
+
* 100pM(100uL) = (200pM)(x)
  
 
x = 50uL in 50uL buffer
 
x = 50uL in 50uL buffer
  
50pM (30uL) = 100pM (x)
+
* 50pM (30uL) = 100pM (x)
  
 
x = 15uL in 15uL
 
x = 15uL in 15uL
Line 70: Line 70:
 
DNA:
 
DNA:
  
5.31uM (500uL) = x (53.1uM)  
+
* 5.31uM (500uL) = x (53.1uM)  
  
 
x = 50uL in 450uL PBS
 
x = 50uL in 450uL PBS
  
5.31uM (x) = 16nM (1000uL)
+
* 5.31uM (x) = 16nM (1000uL)
  
 
x = 3uL in 997uL PBS
 
x = 3uL in 997uL PBS
  
16nM (x) = (8nM) (100uL)
+
* 16nM (x) = (8nM) (100uL)
  
 
x = 50uL in 50uL PBS
 
x = 50uL in 50uL PBS
  
1.92nM (100uL) = x (8nM)
+
* 1.92nM (100uL) = x (8nM)
  
 
x = 24uL in 76 uL PBS
 
x = 24uL in 76 uL PBS
  
0.8nM (100uL) = x (1.92nM)
+
* 0.8nM (100uL) = x (1.92nM)
  
 
x = 41.7uL in 58.3uL
 
x = 41.7uL in 58.3uL
  
160pM (100) = x (800pM)
+
* 160pM (100) = x (800pM)
  
 
x = 20uL in 80uL
 
x = 20uL in 80uL
  
80pM (100uL) = x (160pM)
+
* 80pM (100uL) = x (160pM)
  
 
x = 50uL in 50uL
 
x = 50uL in 50uL
  
40pM (30uL) = x (80pm)
+
* 40pM (30uL) = x (80pm)
  
 
x = 15uL in 15uL PBS
 
x = 15uL in 15uL PBS

Revision as of 08:31, 28 June 2013

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FCS Measurements

  • Run the following samples of MB/DNA

1) 10nM MB + 8nM DNA

2) 5nM MB 4nM DNA

3) 1nM MB 960pM DNA

4) 500pM MB 400pM DNA

5) 100pM MB 80pM DNA

6) 50pM MB 40pM MB

7) 25pM MB 20pM DNA

  • Run the following oligio-D samples

1) 20pM

2) 40pM

3) 60pM

4) 80pM

5) 100pM

Calculations

MOLECULAR BEACON:

  • 7.61μM (x) = 20nM (1000uL)

x = 2.62uL 7.61uM MB in 997.38uL PBS

  • 20nM (x) = (30uL)(10nM)

15uL in 15uL PBS

  • 20nM (x) = 2nM(100uL)

10uL in 90uL

  • 2nM (x) = 1nM (100uL)

x = 50uL in 50uL PBS

  • 200pM (100uL) = x (1nM)

x = 20uL in 80uL PBS

  • 100pM(100uL) = (200pM)(x)

x = 50uL in 50uL buffer

  • 50pM (30uL) = 100pM (x)

x = 15uL in 15uL

DNA:

  • 5.31uM (500uL) = x (53.1uM)

x = 50uL in 450uL PBS

  • 5.31uM (x) = 16nM (1000uL)

x = 3uL in 997uL PBS

  • 16nM (x) = (8nM) (100uL)

x = 50uL in 50uL PBS

  • 1.92nM (100uL) = x (8nM)

x = 24uL in 76 uL PBS

  • 0.8nM (100uL) = x (1.92nM)

x = 41.7uL in 58.3uL

  • 160pM (100) = x (800pM)

x = 20uL in 80uL

  • 80pM (100uL) = x (160pM)

x = 50uL in 50uL

  • 40pM (30uL) = x (80pm)

x = 15uL in 15uL PBS