User:Allison K. Alix/Notebook/Thesis Research/2013/06/13: Difference between revisions
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== | ==MB+DNA Sample Preparation== | ||
Prepare the following samples for FCS Measurements: | |||
1) 100nM thiol-DNA + 120nM MB | |||
2) 1nM DNA + 1.2nM MB | |||
3) 500pM DNA + 600pM MB | |||
4) 100pM DNA + 120pM MB | |||
5) 120nM MB | |||
6) 1.2nM MB | |||
7) 600pM MB | |||
8) 120pM MB | |||
==Calculations== | |||
DNA: (100μL)(200nM)= x(53.1μM) | |||
x= 0.377μL in 99.623μL PBS | |||
MB: (100μL)(240nM) = x(7.61μM) | |||
x= 3.154μL in 96.846μL PBS | |||
1) 15μL 200nM DNA + 15μL 120nM MB | |||
2) (2.4nm)(30μL) = x(240nM) | |||
x= 0.3μL 240nM MB in 29.7μL PBS | |||
(2nM)(30μL) = x(200nM) | |||
x= 0.3μL 200nM DNA in 29.7μL PBS | |||
3) (1000pM)(30μL)= x (2nM) | |||
x= 15μL 2nM DNA in 15μL PBS | |||
(1200pM)(30μL)= x(2.4nmM) | |||
x= 15μL 2.4nM MB in 15μL PBS | |||
4) (200pM)(30μL) = x(1000pM) | |||
x= 6μL 1000pM DNA in 24μL PBS | |||
(240pM)(30μL) = x(1200pM) | |||
x= 6μL 1200pM DNA in 24μL PBS | |||
Revision as of 08:26, 13 June 2013
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MB+DNA Sample PreparationPrepare the following samples for FCS Measurements: 1) 100nM thiol-DNA + 120nM MB 2) 1nM DNA + 1.2nM MB 3) 500pM DNA + 600pM MB 4) 100pM DNA + 120pM MB 5) 120nM MB 6) 1.2nM MB 7) 600pM MB 8) 120pM MB CalculationsDNA: (100μL)(200nM)= x(53.1μM) x= 0.377μL in 99.623μL PBS MB: (100μL)(240nM) = x(7.61μM) x= 3.154μL in 96.846μL PBS 1) 15μL 200nM DNA + 15μL 120nM MB 2) (2.4nm)(30μL) = x(240nM) x= 0.3μL 240nM MB in 29.7μL PBS (2nM)(30μL) = x(200nM) x= 0.3μL 200nM DNA in 29.7μL PBS 3) (1000pM)(30μL)= x (2nM) x= 15μL 2nM DNA in 15μL PBS (1200pM)(30μL)= x(2.4nmM) x= 15μL 2.4nM MB in 15μL PBS 4) (200pM)(30μL) = x(1000pM) x= 6μL 1000pM DNA in 24μL PBS (240pM)(30μL) = x(1200pM) x= 6μL 1200pM DNA in 24μL PBS
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