Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/06/13"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2013/06/13 Entry for User:Allison_K._Alix/Notebook/Thesis_Research)
 
(Entry title)
Line 6: Line 6:
 
| colspan="2"|
 
| colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
+
==MB+DNA Sample Preparation==
* Insert content here...
+
 
 +
Prepare the following samples for FCS Measurements:
 +
 
 +
1) 100nM thiol-DNA + 120nM MB
 +
 
 +
2) 1nM DNA + 1.2nM MB
 +
 
 +
3) 500pM DNA + 600pM MB
 +
 
 +
4) 100pM DNA + 120pM MB
 +
 
 +
5) 120nM MB
 +
 
 +
6) 1.2nM MB
 +
 
 +
7) 600pM MB
 +
 
 +
8) 120pM MB
 +
 
 +
==Calculations==
 +
 
 +
DNA: (100μL)(200nM)= x(53.1μM)
 +
 
 +
x= 0.377μL in 99.623μL PBS
 +
 
 +
MB: (100μL)(240nM) = x(7.61μM)
 +
 
 +
x= 3.154μL in 96.846μL PBS
 +
 
 +
1) 15μL 200nM DNA + 15μL 120nM MB
 +
 
 +
2) (2.4nm)(30μL) = x(240nM)
 +
 
 +
x= 0.3μL 240nM MB in 29.7μL PBS
 +
 
 +
(2nM)(30μL) = x(200nM)
 +
 
 +
x= 0.3μL 200nM DNA in 29.7μL PBS
 +
 
 +
3) (1000pM)(30μL)= x (2nM)
 +
 
 +
x= 15μL 2nM DNA in 15μL PBS
 +
 
 +
(1200pM)(30μL)= x(2.4nmM)
 +
 
 +
x= 15μL 2.4nM MB in 15μL PBS
 +
 
 +
4) (200pM)(30μL) = x(1000pM)
 +
 
 +
x= 6μL 1000pM DNA in 24μL PBS
 +
 
 +
(240pM)(30μL) = x(1200pM)
 +
 
 +
x= 6μL 1200pM DNA in 24μL PBS
  
  

Revision as of 08:26, 13 June 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

MB+DNA Sample Preparation

Prepare the following samples for FCS Measurements:

1) 100nM thiol-DNA + 120nM MB

2) 1nM DNA + 1.2nM MB

3) 500pM DNA + 600pM MB

4) 100pM DNA + 120pM MB

5) 120nM MB

6) 1.2nM MB

7) 600pM MB

8) 120pM MB

Calculations

DNA: (100μL)(200nM)= x(53.1μM)

x= 0.377μL in 99.623μL PBS

MB: (100μL)(240nM) = x(7.61μM)

x= 3.154μL in 96.846μL PBS

1) 15μL 200nM DNA + 15μL 120nM MB

2) (2.4nm)(30μL) = x(240nM)

x= 0.3μL 240nM MB in 29.7μL PBS

(2nM)(30μL) = x(200nM)

x= 0.3μL 200nM DNA in 29.7μL PBS

3) (1000pM)(30μL)= x (2nM)

x= 15μL 2nM DNA in 15μL PBS

(1200pM)(30μL)= x(2.4nmM)

x= 15μL 2.4nM MB in 15μL PBS

4) (200pM)(30μL) = x(1000pM)

x= 6μL 1000pM DNA in 24μL PBS

(240pM)(30μL) = x(1200pM)

x= 6μL 1200pM DNA in 24μL PBS