Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/06/13"

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{|{{table}} width="800"
 
{|{{table}} width="800"
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> DNA/MB Sample Prep</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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x= 3.154μL in 96.846μL PBS
 
x= 3.154μL in 96.846μL PBS
  
1) 15μL 200nM DNA + 15μL 120nM MB
+
1) '''15μL 200nM DNA + 15μL 120nM MB'''
  
 
2) (2.4nm)(30μL) = x(240nM)
 
2) (2.4nm)(30μL) = x(240nM)
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x= 0.3μL 200nM DNA in 29.7μL PBS
 
x= 0.3μL 200nM DNA in 29.7μL PBS
  
15μL 2.4nM MB + 15μL 2nM DNA
+
'''15μL 2.4nM MB + 15μL 2nM DNA'''
  
 
3) (1000pM)(30μL)= x (2nM)
 
3) (1000pM)(30μL)= x (2nM)
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x= 15μL 2.4nM MB in 15μL PBS
 
x= 15μL 2.4nM MB in 15μL PBS
  
15μL 1000pM DNA + 15μL 1200pM MB
+
'''15μL 1000pM DNA + 15μL 1200pM MB'''
  
 
4) (200pM)(30μL) = x(1000pM)
 
4) (200pM)(30μL) = x(1000pM)
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x= 6μL 1200pM DNA in 24μL PBS
 
x= 6μL 1200pM DNA in 24μL PBS
  
15μL 200pM DNA + 15μL 240pM MB
+
'''15μL 200pM DNA + 15μL 240pM MB'''
  
  

Revision as of 10:39, 12 July 2013

Owwnotebook icon.png DNA/MB Sample Prep <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

MB+DNA Sample Preparation

Prepare the following samples for FCS Measurements:

1) 100nM thiol-DNA + 120nM MB

2) 1nM DNA + 1.2nM MB

3) 500pM DNA + 600pM MB

4) 100pM DNA + 120pM MB

5) 120nM MB

6) 1.2nM MB

7) 600pM MB

8) 120pM MB

Calculations

DNA: (100μL)(200nM)= x(53.1μM)

x= 0.377μL in 99.623μL PBS

MB: (100μL)(240nM) = x(7.61μM)

x= 3.154μL in 96.846μL PBS

1) 15μL 200nM DNA + 15μL 120nM MB

2) (2.4nm)(30μL) = x(240nM)

x= 0.3μL 240nM MB in 29.7μL PBS

(2nM)(30μL) = x(200nM)

x= 0.3μL 200nM DNA in 29.7μL PBS

15μL 2.4nM MB + 15μL 2nM DNA

3) (1000pM)(30μL)= x (2nM)

x= 15μL 2nM DNA in 15μL PBS

(1200pM)(30μL)= x(2.4nmM)

x= 15μL 2.4nM MB in 15μL PBS

15μL 1000pM DNA + 15μL 1200pM MB

4) (200pM)(30μL) = x(1000pM)

x= 6μL 1000pM DNA in 24μL PBS

(240pM)(30μL) = x(1200pM)

x= 6μL 1200pM DNA in 24μL PBS

15μL 200pM DNA + 15μL 240pM MB