# Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/06/12"

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2) Stir for 24 hours at room temperature to ensure complete adsorption | 2) Stir for 24 hours at room temperature to ensure complete adsorption | ||

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## Revision as of 10:59, 13 June 2013

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## AuNP synthesis- Make 20mL AuNP using procedure from User:Allison K. Alix/Notebook/Thesis Research/2013/05/10
- Determine concentration and radius using UV-Vis spectrum
Note: These AuNPs will be used in the silica coating procedure. See calculations below. ## Calculating AuNP diameter and concentration (nM)λ d= [ln(λ where λ d= [ln(527nm-512nm/6.53)]/0.0216
c = A c = 1.89/(4.36x10
## Calculating AuNP concentration in g/L1) Calculate the volume of 1 AuNP Volume of 1 AuNP = 4/3πr V= 4/3π(19.25x10 V= 2.988x10
2) Calculate the mass of 1 AuNP density of Au = 19.3g/cm 19.3g/cm 3) Calculate # of AuNP in 20mL solution 0.43x10 4) Calculate mass of all AuNP in 20mL solution 5.7668x10 ## Dilution of AuNPAccording to Reference 1, for the silica coating procedure, 34.7mL AuNP with a radius of ~19nM must have a concentration of 0.058g/L M (0.1505g/L)(x)=(0.058g/L)(37.4mL) x = 13.37mL in 21.33mL H ## Calculating the amount of PVP to add to AuNP- There must be ~60PVP/1nm
^{2}AuNP
1) Calculate the surface area of 1 AuNP Surface Area of a sphere = 4πr SA= 4π(19.25x10 SA= 4.657x10
2) Calculate #PVP per 1 AuNP 60PVP/1nm 3) Calculate the new concentration of AuNP in nM M (0.43x10 x = 1.657x10 4) Calculate # AuNP in 34.7mL 0.1657nM solution 0.1657x10 5) Calculate #moles PVP needed to coat AuNP 279397.5 PVP/AuNP x 3.46x10 6) Calculate concentration needed to add 0.65mL of 1.6065x10 1.6065x10 7) Calculate how many grams 10000g/mol PVP is needed to make 5mL 24.7g/L solution 24.7g/L x 5mL x 1L/1000mL = ## Functionalization of AuNP with PVP1) Mix 34.7mL of 0.058g/L AuNP with 0.65mL 24.7g/L PVP (10kg/mol) 2) Stir for 24 hours at room temperature to ensure complete adsorption ## References |