User:Allison K. Alix/Notebook/Thesis Research/2013/06/12: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Silica Coating Calculations</span> | ||
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Revision as of 11:39, 12 July 2013
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AuNP synthesis
Note: These AuNPs will be used in the silica coating procedure. See calculations below. Calculating AuNP diameter and concentration (nM)λspr= 527nm d= [ln(λspr-λ0/L1)]/L2 where λ0= 512nm, L1= 6.53, and L2=0.0216 (These are fit parameters from theoretical calculations1) d= [ln(527nm-512nm/6.53)]/0.0216 d= 38.5nm c = A450/ε450 c = 1.89/(4.36x109) c = 0.43nM Calculating AuNP concentration in g/L1) Calculate the volume of 1 AuNP Volume of 1 AuNP = 4/3πr3, where r = d/2 = 19.25nm V= 4/3π(19.25x10-9m)3 V= 2.988x1023m3 V= 2.988x1017cm3 2) Calculate the mass of 1 AuNP density of Au = 19.3g/cm3 19.3g/cm3 x 2.988x1017cm3 = 5.7668x10-16 g per 1 AuNP 3) Calculate # of AuNP in 20mL solution 0.43x10-9mol/L x 20mL x 1L/1000mL x 6.02x1023AuNP/1 mol = 5.219x1012 AuNP 4) Calculate mass of all AuNP in 20mL solution 5.7668x10-16 g/1 AuNP x 5.219x1012AuNP x 1/20mL x 1000mL/1L = 0.1505g/L Dilution of AuNPAccording to Reference 1, for the silica coating procedure, 34.7mL AuNP with a radius of ~19nM must have a concentration of 0.058g/L M1V1=M2V2 (0.1505g/L)(x)=(0.058g/L)(37.4mL) x = 13.37mL in 21.33mL H2O Calculating the amount of PVP to add to AuNP
1) Calculate the surface area of 1 AuNP Surface Area of a sphere = 4πr2 SA= 4π(19.25x10-9)2 SA= 4.657x10-15m2 SA= 4656.63nm2 2) Calculate #PVP per 1 AuNP 60PVP/1nm2 x 4656.63nm2/1 AuNP = 279397.5 PVP/1 AuNP 3) Calculate the new concentration of AuNP in nM M1V1=M2V2 (0.43x10-9)(13.37mL) = (x)(34.7mL) x = 1.657x10-10M = 0.1657nM 4) Calculate # AuNP in 34.7mL 0.1657nM solution 0.1657x10-9mol/L x 34.7mL x 1L/1000mL x 6.02x1023AuNP/mol = 3.46x1012 AuNP 5) Calculate #moles PVP needed to coat AuNP 279397.5 PVP/AuNP x 3.46x1012AuNP x 1 mol/6.02x1023PVP = 1.6065x10-6 mol PVP 6) Calculate concentration needed to add 0.65mL of 1.6065x10-6mol PVP 1.6065x10-6 mol PVP x 10000g/mol x 1/0.65mL x 1000mL/L = 24.7g/L 7) Calculate how many grams 10000g/mol PVP is needed to make 5mL 24.7g/L solution 24.7g/L x 5mL x 1L/1000mL = 0.1235g in 5mL H2O Functionalization of AuNP with PVP1) Mix 34.7mL of 0.058g/L AuNP with 0.65mL 24.7g/L PVP (10kg/mol) 2) Stir for 24 hours at room temperature to ensure complete adsorption References1) [1] with supporting information [2]
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