User:Allison K. Alix/Notebook/Thesis Research/2013/05/14: Difference between revisions
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Calculations: | |||
Part 1: Preparing 17.8μM thiol-DNA | |||
original concentration of thiol-DNA- 53.1μM | |||
53.1μM (x) = (17.8μM)(500μL) | |||
x= 167.63μL in 332.37μL buffer | |||
Part 2: Preparing 20μM ThT | |||
original conc ThT= 5mM | |||
5mM (x) = (0.02mM)(10mL) | |||
x=40μL in 9960μL H<sub>2</sub>O | |||
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Revision as of 07:50, 14 May 2013
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Objectives
Procedures
Previous concentrations used:
To be used:
Calculations: Part 1: Preparing 17.8μM thiol-DNA original concentration of thiol-DNA- 53.1μM 53.1μM (x) = (17.8μM)(500μL) x= 167.63μL in 332.37μL buffer Part 2: Preparing 20μM ThT original conc ThT= 5mM 5mM (x) = (0.02mM)(10mL) x=40μL in 9960μL H2O |