User:Allison K. Alix/Notebook/Thesis Research/2013/05/10: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Objectives== | ||
* | |||
* Take absorbance and fluorescence spectra of thiol DNA/ThT/AuNP solutions | |||
* If signals are still exhibited, run 2% agarose gel on concentrated sample as well as supernatants | |||
* Synthesize AuNP | |||
==Procedures== | |||
'''Synthesizing AuNP''' | |||
1) Stir and heat to boiling 20mL of 1mM HAuCl<sub>4</sub> | |||
2) Add 2mL 1% sodium citrate | |||
3) Observe a purple to red color change | |||
4) Determine size and concentration of AuNP using UV-Vis | |||
==Data== | |||
[[Image:Fluorescence_Spec_Pic.png]] | |||
==Observations== | |||
Some fluorescence was seen in the thiol DNA/ThT/AuNP sample as shown in the spectrum above. Because it is such a small amount of fluorescence, we are interested in trying this procedure at one magnitude in concentration higher than previously used (of all components). Gold nanoparticles specifically will need to be re-synthesized at a higher concentration. | |||
Latest revision as of 22:41, 26 September 2017
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Objectives
ProceduresSynthesizing AuNP 1) Stir and heat to boiling 20mL of 1mM HAuCl4 2) Add 2mL 1% sodium citrate 3) Observe a purple to red color change 4) Determine size and concentration of AuNP using UV-Vis Data
ObservationsSome fluorescence was seen in the thiol DNA/ThT/AuNP sample as shown in the spectrum above. Because it is such a small amount of fluorescence, we are interested in trying this procedure at one magnitude in concentration higher than previously used (of all components). Gold nanoparticles specifically will need to be re-synthesized at a higher concentration.
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