Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/04/23"

From OpenWetWare
Jump to: navigation, search
Line 35: Line 35:
Part 3: React thiol-DNA/ThT with AuNP
Part 3: React thiol-DNA/ThT with AuNP
Use procedure from [[User:Allison K. Alix/Notebook/Thesis Research/2013/04/15|04/15/2013]]

Revision as of 09:15, 23 April 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


  • Run 2% Gel with DNA-AuNP samples and supernatants
  • Measure abs/fluor of thiol DNA/ThT
  • Attach AuNP to thiol DNA/ThT


Part 1:

Run 2% agarose gel with the following samples:

1)5uL 100bp ladder + 1uL loading buffer

2)5uL DNA-AuNP + 1uL loading buffer

3)5uL sup 1 + 1uL loading buffer

4)5uL sup 2 + 1uL loading buffer

5)5uL sup 3 + 1uL loading buffer

6)5uL sup 4 + 1uL loading buffer

Part 2: Take Absorbance and Fluorescence of thiol DNA-ThT sample

Part 3: React thiol-DNA/ThT with AuNP

Use procedure from 04/15/2013