Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/04/23"

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(Procedures)
(Objectives)
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==Objectives==
 
==Objectives==
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*Run 2% Gel with DNA-AuNP samples and supernatants
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*Measure abs/fluor of thiol DNA/ThT
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*Attach AuNP to thiol DNA/ThT
  
 
==Procedures==
 
==Procedures==

Revision as of 08:08, 23 April 2013

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Objectives

  • Run 2% Gel with DNA-AuNP samples and supernatants
  • Measure abs/fluor of thiol DNA/ThT
  • Attach AuNP to thiol DNA/ThT

Procedures

Part 1:

Run 2% agarose gel with the following samples:

1)5uL 100bp ladder + 1uL loading buffer

2)5uL DNA-AuNP + 1uL loading buffer

3)5uL sup 1 + 1uL loading buffer

4)5uL sup 2 + 1uL loading buffer

5)5uL sup 3 + 1uL loading buffer

6)5uL sup 4 + 1uL loading buffer

Part 2: Take Absorbance and Fluorescence of thiol DNA-ThT sample

Part 3: React thiol-DNA/ThT with AuNP