Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/04/16"

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(Procedures)
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==Procedures==
 
==Procedures==
 +
 +
Part one: Reconstituting samples in water
 +
 +
1) Remove epi-test tubes from lyophilizer
 +
 +
2) Add 10 μL water to each sample and vortex to dissolve
 +
 +
Part 2: Gel Electrophoresis.
 +
 +
1) Follow procedure outlined on
  
 
53.1micM DNA x = 500micL (5.82micM)
 
53.1micM DNA x = 500micL (5.82micM)

Revision as of 09:28, 16 April 2013

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Objectives

  • Reconstitute lyophilized DNA samples in 10 μL water and run gel.
  • Take absorbance spectra of thiol-DNA/ThT/AuNP sample and supernatants
  • Prepare thiol-DNA/ThT solution with higher concentrations of DNA

Procedures

Part one: Reconstituting samples in water

1) Remove epi-test tubes from lyophilizer

2) Add 10 μL water to each sample and vortex to dissolve

Part 2: Gel Electrophoresis.

1) Follow procedure outlined on

53.1micM DNA x = 500micL (5.82micM)

54.8micL in 445.2 mic buffer