Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/04/16"
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Part one: Reconstituting DNA samples in water
1) Remove epi-test tubes from lyophilizer
2) Add 10 μL water to each sample and vortex to dissolve
Part 2: Gel Electrophoresis.
1) Follow procedure outlined on 03/29/2013
2) Prepare the following 7 samples:
3) Load into gel
4) Stain with ehtidium bromide
5) Observe under UV Light
Part 3: Preparing thiol-DNA/ThT solution
1) Dilute stock DNA (53.1μM) to 5.82μM
53.1μM DNA (x) = (500μL) (5.82μM)
X = 54.8micL in 445.2 mic buffer
2) Mix 250μL thiol-DNA with 250μL 5μMThT
3) Heat at ~75°C for 25 minutes
4) Cool to room temperature
The absorbance data did not show a trend. The thiol-DNA/ThT/AuNP sample itself was the faintest shade of purple, which may be one reason we did not observe a distinguishable peak. Because of this, we decided to re-do the thiol-DNA/ThT reaction using a higher concentration of DNA (See Part 3 of the procedures). This allowed us to work with a higher concentration of gold nanoparticles since it's concentration is based off of the concentration of DNA used.