Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/04/11"

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==Procedures==
 
==Procedures==
  
Part 1: Gel Electrophoresis on DNA-AuNP hybrid
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'''Part 1: Gel Electrophoresis on DNA-AuNP hybrid'''
  
 
* Follow procedures outlined on [[User:Allison K. Alix/Notebook/Thesis Research/2013/03/29|03/29/2013]]
 
* Follow procedures outlined on [[User:Allison K. Alix/Notebook/Thesis Research/2013/03/29|03/29/2013]]
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6) 4μL filtered water + 1μL supernatant 4 + 1μL loading dye
 
6) 4μL filtered water + 1μL supernatant 4 + 1μL loading dye
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 +
* Stain for ~10min in ethidium bromide
  
 
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Revision as of 08:25, 12 April 2013

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Objectives

  • Complete Gel Electrophoresis on DNA-AuNP hybrid synthesized 3/18
  • Observe under UV light

Procedures

Part 1: Gel Electrophoresis on DNA-AuNP hybrid

The following samples were observed

1) 4μL filtered water + 1μL 100bp DNA ladder + 1μL loading dye

2) 4μL filtered water + 1μL DNA/AuNP + 1μL loading dye

3) 4μL filtered water + 1μL supernatant 1 + 1μL loading dye

4) 4μL filtered water + 1μL supernatant 2 + 1μL loading dye

5) 4μL filtered water + 1μL supernatant 3 + 1μL loading dye

6) 4μL filtered water + 1μL supernatant 4 + 1μL loading dye

  • Stain for ~10min in ethidium bromide