User:Allison K. Alix/Notebook/Thesis Research/2013/04/09: Difference between revisions
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* react 200μL 298μM ethane thiol with 200μL 19.4nM AuNP (synthesized by Dr. Miller) | * react 200μL 298μM ethane thiol with 200μL 19.4nM AuNP (synthesized by Dr. Miller) | ||
Part 2: Measure absorbance/fluorescence of AuNP-MB | '''Part 2: Measure absorbance/fluorescence of AuNP-MB''' | ||
Part 3: Measure absorbance/fluorescence of AuNP-ThT | '''Part 3: Measure absorbance/fluorescence of AuNP-ThT''' | ||
Revision as of 07:49, 9 April 2013
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Objectives
ProceduresPart 1: Preparation of 1mL of 2.98μM ethane thiol WARNING: ethanethiol is very volatile. Handle under the hood only wearing PPE.
1) Calculating concentration of ethanethiol 0.839g/mL x 0.025mL = 0.020975g 0.020975g/(62.13 g/mol) = 3.376 x 10-4 mol 3.376 x 10-4 mol/1mL x (1000mL/1L) = 0.34M = 340mM 340mM (x) = (30mM)(1mL) x = 0.088mL = 88μL in 912μL H2O 30mM (x) = 3mM (1mL) x = 100μL in 900μL 3mM(x) = 298μM (1mL) x = 99μL in 901μL to obtain 298μM ethanethiol 2) Reacting AuNP with ethanethiol
Part 2: Measure absorbance/fluorescence of AuNP-MB Part 3: Measure absorbance/fluorescence of AuNP-ThT
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