Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/04/05"

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==Procedures==
 
==Procedures==
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Part 1: Mixing thiol-DNA with ThT
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 +
1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA
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 +
* Note that new concentrations of reactants in solution are 2.5μM ThT and 0.7275μM DNA
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 +
2) Place on heat block at ~76°C for 25 minutes
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 +
3) Allow solution to cool on heat block until at room temperature
 +
 +
4) Take absorbance and fluorescence measurements
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 +
* AuNP will then be added to this solution
  
  

Revision as of 06:00, 5 April 2013

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Objectives

  • Mix DNA with higher concentration of ThT
  • Measure absorbance and fluorescence of DNA/ThT hybrid

Procedures

Part 1: Mixing thiol-DNA with ThT

1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA

  • Note that new concentrations of reactants in solution are 2.5μM ThT and 0.7275μM DNA

2) Place on heat block at ~76°C for 25 minutes

3) Allow solution to cool on heat block until at room temperature

4) Take absorbance and fluorescence measurements

  • AuNP will then be added to this solution