User:Allison K. Alix/Notebook/Thesis Research/2013/04/04

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Owwnotebook icon.png Abs and Fluor Measurements of thiol-DNA/ThT <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objectives

  • Measure absorbance and fluorescence of thiol-DNA/MB and thiol-DNA/ThT mixtures prepared on 04/02/2013
  • React both with AuNP

Procedures

Part 1: Measure absorbance values of hybrid

thiol-DNA/ThT

fluorescence:

1) excitation at 400. Start 410, end 610 (according to absorbance spec

2) excitation at 440 start at 450 end 650

3) excitation at 417 start at 427 end 650

Part 2: Measure fluorescence of ThT and DNA alone

Observations

  • The concentration of ThT used in this solution was likely too low. Although ThT was in excess, the absorbance seen on the UV-Vis spectrum was very low. It is interesting to note that the DNA-ThT hybrid did show slight fluorescence intensity. Because there is a possibility that this signal was a background signal coming from the ThT, a fluorescence spectrum of that same concentration of ThT alone in solution was also taken (See Data below)

Data

Absorbance:

ThiolDNAThThybridUVVisspec.png

Fluorescence:

ThiolDNAThThybridFluorescencespec.png