Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/04/04"

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(Procedures)
(Procedures)
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==Procedures==
 
==Procedures==
  
Part 1: Measure absorbance values
+
Part 1: Measure absorbance values of hybrid
  
 
thiol-DNA/ThT
 
thiol-DNA/ThT
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3) excitation at 417 start at 427 end 650
 
3) excitation at 417 start at 427 end 650
 +
 +
Part 2: Measure fluorescence of ThT and DNA alone
 +
 +
==Observations==
 +
 +
  
  

Revision as of 06:01, 5 April 2013

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Objectives

  • Measure absorbance and fluorescence of thiol-DNA/MB and thiol-DNA/ThT mixtures prepared on 04/02/2013
  • React both with AuNP

Procedures

Part 1: Measure absorbance values of hybrid

thiol-DNA/ThT

fluorescence:

1) excitation at 400. Start 410, end 610 (according to absorbance spec

2) excitation at 440 start at 450 end 650

3) excitation at 417 start at 427 end 650

Part 2: Measure fluorescence of ThT and DNA alone

Observations