Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/04/02"

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(Entry title)
(Procedures)
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==Procedures==
 
==Procedures==
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Part 1: Reacting DNA with MB first, followed by a reaction of the DNA-MB hybrid with AuNP
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1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB
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 +
* 727.5nm 8-repeat DNA-thiol
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 +
(Original concentration of DNA is 53.1μM)
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 +
53.1μM(x)=(500μL)(1455nm)
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 +
x= 13.7μL in 486.3μL H<sub>2</sub>O
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 +
1455nm(x) =
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 +
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Revision as of 07:57, 2 April 2013

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Objectives

  • Bind DNA to MB and AuNP
  • React AuNP with ethanethiol

Procedures

Part 1: Reacting DNA with MB first, followed by a reaction of the DNA-MB hybrid with AuNP

1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB

  • 727.5nm 8-repeat DNA-thiol

(Original concentration of DNA is 53.1μM)

53.1μM(x)=(500μL)(1455nm)

x= 13.7μL in 486.3μL H2O

1455nm(x) =