Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/02/12"

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*Take Fluorescence Measurements of samples from [[User:Allison_K._Alix/Notebook/Thesis_Research/2013/02/05|2/5/2013]]
 
*Take Fluorescence Measurements of samples from [[User:Allison_K._Alix/Notebook/Thesis_Research/2013/02/05|2/5/2013]]
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* We are expecting to see a decrease in signal with decreasing DNA concentration
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=Spectra=
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[[Image:4repeatDNAThTfluorgraph.jpg]]
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[[Image:8repeatDNAThTfluorgraph.jpg]]
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[[Image:Fluorintcomp1.5micM.png‎]]
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[[Image:Fluorintcomp2.0micM.png‎]]
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[[Image:Fluorintcomp2.5micM.png‎]]
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[[Image:Fluorintcomp3.0micM.png‎]]
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[[Image:Fluorintcomp3.5micM.png‎]]
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=Observations=
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4 repeat telomeric DNA samples:
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There was only a trend of increasing intensity with increasing concentration of DNA seen in the 1.5μM, 3.0μM, and 3.5μM samples. The 2.0μM and 2.5μM samples were very much out of order. Next step is to plot intensity at max peak vs concentration.
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[[Image:Fluorintvsconc4strandpic.png]]
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[[Image:Fluorintvsconc8strandpic.png]]
  
 
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Revision as of 10:15, 19 February 2013

Owwnotebook icon.png Fluorescence Measurements <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objectives

  • Take Fluorescence Measurements of samples from 2/5/2013
  • We are expecting to see a decrease in signal with decreasing DNA concentration

Spectra

4repeatDNAThTfluorgraph.jpg

8repeatDNAThTfluorgraph.jpg

Fluorintcomp1.5micM.png

Fluorintcomp2.0micM.png

Fluorintcomp2.5micM.png

Fluorintcomp3.0micM.png

Fluorintcomp3.5micM.png

Observations

4 repeat telomeric DNA samples:

There was only a trend of increasing intensity with increasing concentration of DNA seen in the 1.5μM, 3.0μM, and 3.5μM samples. The 2.0μM and 2.5μM samples were very much out of order. Next step is to plot intensity at max peak vs concentration.


Fluorintvsconc4strandpic.png

Fluorintvsconc8strandpic.png