Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/01/15"

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(Re-running Fluorescence Measurements)
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==Re-running Fluorescence Measurements==
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==Procedures==
* Fluorescence Spectra for the following samples were obtained:
 
  
*concentrated AuNP - (3x10^-9 M)excited at 515nm between 530 and 680
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Obtain fluorescence spectra for the following samples:(excited at 515nm)
  
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* concentrated AuNP (3x10^-9 M)
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* 1:6 diluted AuNP (6.49 x10^-9 M)
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* 1:6 diluted AuNP with thiol primer attached. (10μL diluted to 100μL with 90μL water)
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* Elaine's Thiol Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\
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* Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O)
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Telomerase Molecular Beacon was diluted with 1mL filtered H<sub>2</sub>O, separated into vials in 10μL aliquots and placed in the freezer.
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==Data==
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[[Image:FluorSpecAll.jpg]]
  
 
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Revision as of 12:14, 15 January 2013

Owwnotebook icon.png Fluorescence Measurements <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Procedures

Obtain fluorescence spectra for the following samples:(excited at 515nm)

  • concentrated AuNP (3x10^-9 M)
  • 1:6 diluted AuNP (6.49 x10^-9 M)
  • 1:6 diluted AuNP with thiol primer attached. (10μL diluted to 100μL with 90μL water)
  • Elaine's Thiol Primer (10μL diluted to 100μL with 90μL H2O)\
  • Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H2O)

Telomerase Molecular Beacon was diluted with 1mL filtered H2O, separated into vials in 10μL aliquots and placed in the freezer.

Data

FluorSpecAll.jpg