Difference between revisions of "User:Allison K. Alix/Notebook/Fluorescence Detection of Telomerase Activity/2012/11/15"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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-take fluorescence measurements of several mixtures all of which contain cancer cell solution
 
-take fluorescence measurements of several mixtures all of which contain cancer cell solution
  
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==Procedure==
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Preparation of Mixtures:
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 +
1) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL telomerase complementary strand, 25μL Molecular Beacon
 +
  b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL telomerase complementary strand, 25μL Molecular Beacon
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2) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL Molecular Beacon
 +
  b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL Molecular Beacon
 +
 +
3) 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 10μL 5μM Zinc Protoporphyrin IX
 +
 +
4) 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 10μL 5μM Zinc Protoporphyrin IX
 +
 +
5) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL telomerase complementary strand
 +
  b. b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL telomerase complementary strand
  
 
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Latest revision as of 22:15, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page

Objectives

-take fluorescence measurements of several mixtures all of which contain cancer cell solution


Procedure

Preparation of Mixtures:

1) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL telomerase complementary strand, 25μL Molecular Beacon

  b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL telomerase complementary strand, 25μL Molecular Beacon

2) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL Molecular Beacon

  b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL Molecular Beacon

3) 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 10μL 5μM Zinc Protoporphyrin IX

4) 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 10μL 5μM Zinc Protoporphyrin IX

5) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL telomerase complementary strand

  b. b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL telomerase complementary strand