User:Allison K. Alix/Notebook/CHEM-581/2013/04/12: Difference between revisions

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5) Take absorbance of remaining filtrant
5) Take absorbance of remaining filtrant


6) Redisperse hydrogels on filter paper back into phosphate buffer and observe under microscope
6) Redisperse hydrogels on filter paper back into 10 mL phosphate buffer and observe under microscope


Part 2:
Part 2: Mixing ultrasonicated hydrogels
 
The following samples were prepared:
 
*


==Data==
==Data==

Revision as of 12:39, 12 April 2013

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Objectives

  • Sonicate hydrogels containing dye using ultra sonic liquid processing and filter (2x)
  • Mix sonicated hydrogels containing no dye with dye and indicators to monitor absorbance

Procedures

Part 1: Sonication of Hydrogels

1) Measure ~7.5mL of solution C

2) Sonicate for ~5 minutes at 15 watts

3) Filter using vacuum filtration (Filter used: Quantitative P8, Porosity: Coarse, Flow Rate: Fast)

4) Filter the filtrant once more through a nylon filter.

5) Take absorbance of remaining filtrant

6) Redisperse hydrogels on filter paper back into 10 mL phosphate buffer and observe under microscope

Part 2: Mixing ultrasonicated hydrogels

The following samples were prepared:

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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