User:Allison K. Alix/Notebook/CHEM-581/2013/04/03: Difference between revisions

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*Dilute 200μL of solution 4 with 200μL phosphate buffer (solution 5)
*Dilute 200μL of solution 4 with 200μL phosphate buffer (solution 5)


2) Dilute 2mg of hydrogels containing dye in 1.5mL phosphate buffer (from 3/22/13) (marked with heart)
2) Dilute 200μL 2mg of hydrogel solution containing dye in 1.5mL phosphate buffer (from 3/22/13) (marked with heart) with 200μL phosphate buffer (solution 1)
 
*Dilute 200μL of solution 1 with 200μL phosphate buffer (solution 2)
*Dilute 200μL of solution 2 with 200μL phosphate buffer (solution 3)
*Dilute 200μL of solution 3 with 200μL phosphate buffer (solution 4)
*Dilute 200μL of solution 4 with 200μL phosphate buffer (solution 5)
 
Part 4: Preparation of hydrogels
 
1) Prepare the following:
 
*2 samples of 0.5g (89,000-98,000 g/mol) PVOH in 6mL phosphate buffer
*2 samples of 0.5g (89,000-98,000 g/mol) PVOH in 6mL phosphate buffer with 50μL 1mM R6G
 
2) Heat solutions at ~110°C for 15 minutes. Allow to cool to room temperature.
 
3) mix with 50mL safflower oil
 
4) Freeze with liquid nitrogen and place in freezer at -20°C for 24 hours


==Data==
==Data==

Revision as of 07:56, 3 April 2013

Experimental Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

-Mix hydrogels with remaining indicators (bromocresol purple, phenolphthalein, cresol red)

-View hydrogels containing fluorescent dye (R6G) under fluorescent microscope

-Prepare new sets of hydrogels

Procedures

Part 1: Addition of indicators to hydrogel solutions

1) Add 50μL of phenolphthalein to 5mg hydrogel in 1.5mL buffer prepared on 03/29/2013

2) Add 25μL bromocresol purple to 5mg hydrogels in 1.5mL phosphate buffer

3) Add 75μL 0.02% cresol red to 5mg hydrogels in 1.5mL phosphate buffer

4) Allow time for hydrogels to absorb indicator

Part 2: Create concentration gradient for fluroescence calibration of microscope

1) Prepare a solution of 10μL FluoSpheres carboxylate modified microspheres, (0.1μM, orange fluorescent (540/560) 2% solids) in 100μL distilled water (solution 1) 0.2%

2) Prepare the following subsequent dilutions

  • solution 2: 10μL solution 1 in 100μL distilled water 0.02%
  • solution 3: 10μL solution 2 in 100μL distilled water 0.002%
  • solution 4: 10μL solution 3 in 100μL distilled water 0.0002%

Part 3: Preparing dilutions of dye containing hydrogels (marked with *)

1) Dilute 2mg hydrogels containing 30μL R6G (supernatant was previously removed) with 400μL phosphate buffer (solution 1)

  • Dilute 200μL of solution 1 with 200μL phosphate buffer (solution 2)
  • Dilute 200μL of solution 2 with 200μL phosphate buffer (solution 3)
  • Dilute 200μL of solution 3 with 200μL phosphate buffer (solution 4)
  • Dilute 200μL of solution 4 with 200μL phosphate buffer (solution 5)

2) Dilute 200μL 2mg of hydrogel solution containing dye in 1.5mL phosphate buffer (from 3/22/13) (marked with heart) with 200μL phosphate buffer (solution 1)

  • Dilute 200μL of solution 1 with 200μL phosphate buffer (solution 2)
  • Dilute 200μL of solution 2 with 200μL phosphate buffer (solution 3)
  • Dilute 200μL of solution 3 with 200μL phosphate buffer (solution 4)
  • Dilute 200μL of solution 4 with 200μL phosphate buffer (solution 5)

Part 4: Preparation of hydrogels

1) Prepare the following:

  • 2 samples of 0.5g (89,000-98,000 g/mol) PVOH in 6mL phosphate buffer
  • 2 samples of 0.5g (89,000-98,000 g/mol) PVOH in 6mL phosphate buffer with 50μL 1mM R6G

2) Heat solutions at ~110°C for 15 minutes. Allow to cool to room temperature.

3) mix with 50mL safflower oil

4) Freeze with liquid nitrogen and place in freezer at -20°C for 24 hours

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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