Difference between revisions of "User:Allison K. Alix/Notebook/CHEM-581/2013/03/27"

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==Objective==
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==Objectives==
  
 
-Convert bound ULVs into a single bilayer coating the microgels
 
-Convert bound ULVs into a single bilayer coating the microgels
  
 
-Observe hydrogels containing dye under microscope
 
-Observe hydrogels containing dye under microscope
 +
 +
-Prepare hydrogels containing new pH sensitive indicators
  
 
==Procedures==
 
==Procedures==
  
Part 1:
+
'''Part 1: Finish preparing liposome coated hydrogels'''
  
1) Complete five freeze-thaw vortexes using liquid nitrogen (Freeze for 30 seconds or until frozen, thaw for 30 seconds or until thawed and vortex for 30 seconds. Repeat)
+
1) Complete five freeze-thaw vortexes using liquid nitrogen on hydrogel-liposome solution(Freeze for 30 seconds or until frozen, thaw for 30 seconds or until thawed and vortex for 30 seconds. Repeat)
  
 
2) Remove unbound ULVs by centrifuging at 4000 rpm for 5 minutes
 
2) Remove unbound ULVs by centrifuging at 4000 rpm for 5 minutes
  
3) Resuspend in tris-HCl buffer
+
3) Resuspend in 25mM tris-HCl buffer
 +
 
 +
'''Part 2: Preparing a concentration gradient of R6G in hydrogels'''
 +
 
 +
1) Add the following volumes of 1mM R6G to 2mg PVOH hydrogels in 1.5mL phosphate buffer:
 +
 
 +
10μL, 15μL, 20μL, 25μL, 30μL, 35μL
 +
 
 +
2) Allow absorption of dye for 48 hours
 +
 
 +
'''Part 3: Preparing pH sensitive hydrogels'''
 +
 
 +
1) Centrifuge hydrogel solutions containing methyl red and bromophenol blue prepared on 3/22
 +
 
 +
2) Collect supernatant and add 1mL phosphate buffer
 +
 
 +
3) Repeat to colelct a total of 3 supernatants for each solution
 +
 
 +
4) Collect UV-Vis spectra for each supernatant as well as stock solution to observe the amount of dye absorbed by the hydrogel
 +
 
 +
5) Observe pH changes of hydrogels w/ indicator.
  
 
==Data==
 
==Data==
* Add data and results here...
+
 
 +
*We were not able to view the hydrogels under the microscope (maintenance issues) This will be completed at antoher time.
  
 
==References==
 
==References==
 
Lipogels: Single-Lipid-Bilayer-Enclosed Hydrogel Spheres
 
Lipogels: Single-Lipid-Bilayer-Enclosed Hydrogel Spheres
 
Qasim Saleem,†,§,|| Baoxu Liu,§,‡,||Claudiu C. Gradinaru,*,§,‡ and Peter M. Macdonald
 
Qasim Saleem,†,§,|| Baoxu Liu,§,‡,||Claudiu C. Gradinaru,*,§,‡ and Peter M. Macdonald
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
 
[[Category:Course]]
 
[[Category:Miscellaneous]]
 
 
  
  

Revision as of 07:17, 27 March 2013

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Objectives

-Convert bound ULVs into a single bilayer coating the microgels

-Observe hydrogels containing dye under microscope

-Prepare hydrogels containing new pH sensitive indicators

Procedures

Part 1: Finish preparing liposome coated hydrogels

1) Complete five freeze-thaw vortexes using liquid nitrogen on hydrogel-liposome solution(Freeze for 30 seconds or until frozen, thaw for 30 seconds or until thawed and vortex for 30 seconds. Repeat)

2) Remove unbound ULVs by centrifuging at 4000 rpm for 5 minutes

3) Resuspend in 25mM tris-HCl buffer

Part 2: Preparing a concentration gradient of R6G in hydrogels

1) Add the following volumes of 1mM R6G to 2mg PVOH hydrogels in 1.5mL phosphate buffer:

10μL, 15μL, 20μL, 25μL, 30μL, 35μL

2) Allow absorption of dye for 48 hours

Part 3: Preparing pH sensitive hydrogels

1) Centrifuge hydrogel solutions containing methyl red and bromophenol blue prepared on 3/22

2) Collect supernatant and add 1mL phosphate buffer

3) Repeat to colelct a total of 3 supernatants for each solution

4) Collect UV-Vis spectra for each supernatant as well as stock solution to observe the amount of dye absorbed by the hydrogel

5) Observe pH changes of hydrogels w/ indicator.

Data

  • We were not able to view the hydrogels under the microscope (maintenance issues) This will be completed at antoher time.

References

Lipogels: Single-Lipid-Bilayer-Enclosed Hydrogel Spheres Qasim Saleem,†,§,|| Baoxu Liu,§,‡,||Claudiu C. Gradinaru,*,§,‡ and Peter M. Macdonald