Difference between revisions of "User:Allison K. Alix/Notebook/CHEM-581/2013/03/22"

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
 
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Description==
 
==Description==
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1) Centrifuge sample containing hydrogels and crystal violet indicator 3 times (5 minutes, 13200 rpm)
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-Measure the absorbance of each supernatant to find the amount of CV that the hydrogels have absorbed
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2) Prepare 2 more hydrogel solutions with the following indicators (these will be used to measure dye leakage as well as to monitor pH changes)
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a) methyl red (55μL) in 1.5 mL ethanol w/ 2mg hydrogels
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b) bromophenol blue (30μL) in 1.5 mL water w/ 2mg hydrogels
  
 
==Data==
 
==Data==
* Add data and results here...
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[[Image:Crystal violet leakage.png]]
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This image shows the absorbance of the stock solution of crystal violet that was originally added to the hydrogels as well as three "leakage" solutions. The hydrogel solution containing CV was centrifuged 3 times to get each successive leakage. From this image we can observe a decreasing absorbance beginning with the stock solution and ending with the third leakage solution. This makes sense as the first supernatant should have the most (if not all) excess CV not absorbed by the hydrogel.
  
 
==Notes==
 
==Notes==
This area is for any observations or conclusions that you would like to note.
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Our next step is to look at a hydrogel solution containing dye under a microscope.
 
 
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
  
 
[[Category:Course]]
 
[[Category:Course]]

Latest revision as of 21:34, 26 September 2017

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Objective

-mix hydrogels with liposomes

-Measure amount of indicator absorbed by hydrogels

-observe changes in color of hydrogels based on pH changes

Description

1) Centrifuge sample containing hydrogels and crystal violet indicator 3 times (5 minutes, 13200 rpm)

-Measure the absorbance of each supernatant to find the amount of CV that the hydrogels have absorbed

2) Prepare 2 more hydrogel solutions with the following indicators (these will be used to measure dye leakage as well as to monitor pH changes)

a) methyl red (55μL) in 1.5 mL ethanol w/ 2mg hydrogels

b) bromophenol blue (30μL) in 1.5 mL water w/ 2mg hydrogels

Data

Crystal violet leakage.png

This image shows the absorbance of the stock solution of crystal violet that was originally added to the hydrogels as well as three "leakage" solutions. The hydrogel solution containing CV was centrifuged 3 times to get each successive leakage. From this image we can observe a decreasing absorbance beginning with the stock solution and ending with the third leakage solution. This makes sense as the first supernatant should have the most (if not all) excess CV not absorbed by the hydrogel.

Notes

Our next step is to look at a hydrogel solution containing dye under a microscope.