Difference between revisions of "User:Allison K. Alix/Notebook/CHEM-581/2013/03/18"

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(Procedure)
(Procedure)
 
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==Procedure==
 
==Procedure==
  
1) Dissolve 100mg transfection agent in 5mL chloroform
+
1) Dissolve 100mg transfection reagent in 5mL chloroform
 +
 
 +
2) Remove 1mL of solution and place in a seperate vial. Allow to evaporate.
 +
 
 +
3) Once evaporated, add 1mL phosphate buffer to redissolve lipids
 +
 
 +
4) 3 freeze-thaw-vortex cycles were applied to the solution to create the multilameller vesicles
 +
 
 +
5) These multilamellar vesicles were then converted to unilamellar vesicles by passing through a nucleopore membrane
 +
 
  
2) remove 1mL of solution and place in a seperate vial. Allow to evaporate.
 
  
 
indicators
 
indicators

Latest revision as of 08:45, 27 March 2013

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Objective

Prepare Lipid Bilayer

Procedure

1) Dissolve 100mg transfection reagent in 5mL chloroform

2) Remove 1mL of solution and place in a seperate vial. Allow to evaporate.

3) Once evaporated, add 1mL phosphate buffer to redissolve lipids

4) 3 freeze-thaw-vortex cycles were applied to the solution to create the multilameller vesicles

5) These multilamellar vesicles were then converted to unilamellar vesicles by passing through a nucleopore membrane


indicators

methyl violet 0-2

methyl red- 4.4-6.2

bromocresol purple- 5.2-6.8

bromophenol blue 6.0-7.6

cresol red 7.2-8.8

phenolphthalein- 8.3-10.0

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.