User:Allison K. Alix/Notebook/CHEM-581/2013/03/08

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BDLlogo notext lr.png Measuring Dye Absorbance <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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-Suspend hydrogels back into solution

-Attach dye to hydrogels once they are in solution

-Measure the amount of dye absorbed


Part 1

1) Add 0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer

2) Sonicate for ~25 minutes or until hydrogels have separated

3) Add 5 μL of dye.

4) Allow hydrogels to absorb dye

5) Measure the absorbance of each solution in 15 minute intervals (1mL aliquots)

6) Add 1 mL distilled water for every 1mL taken out of solution

Part 2

1) Place 2mg of PVOH (w/ R6G, w/o lecithin) in 1.5mL of the following solutions:

a) distilled water
b) phosphate buffer
c) 1M NaOH
d) 1M HCl
e) glutaraldehyde fixant solution (NaCl)

2) Allow hydrogels to stay in solution for 1 hour

3) Centrifuge for 5 minutes

4) Take absorbance measurements


  • Add data and results here...


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