User:Allison K. Alix/Notebook/CHEM-581/2013/02/20

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Objective

  • Use Safflower Oil in place of Mineral oil
  • Freeze using Liquid Nitrogen before placing in freezer

Procedures

Make the following samples:

1) 0.5g PVOH, 4 mL buffer, 50 mL oil

2) 0.25g PVOH, 3 mL buffer, 50 mL oil

3) 0.14g PVOH, 3mL buffer, 50 mL oil

Set 1:

Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

Set 2:

Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

Set 3:

Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing

Data

Notes


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