Difference between revisions of "User:Allison K. Alix/Notebook/CHEM-581/2013/02/20"

From OpenWetWare
Jump to: navigation, search
(fix raw html notebook nav)
 
(8 intermediate revisions by one other user not shown)
Line 1: Line 1:
 
{|{{table}} width="800"
 
{|{{table}} width="800"
 
|-
 
|-
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Hydrogel Prep III</span>
+
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Microgel Prep III</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
Line 11: Line 11:
 
==Objective==
 
==Objective==
  
* Use Saffron Oil in place of Mineral oil
+
* Use Safflower Oil (higher freezing point than mineral oil)
  
 
* Freeze using Liquid Nitrogen before placing in freezer
 
* Freeze using Liquid Nitrogen before placing in freezer
  
==Description==
+
==Procedures==
  
 
Make the following samples:
 
Make the following samples:
Line 25: Line 25:
 
3) 0.14g PVOH, 3mL buffer, 50 mL oil
 
3) 0.14g PVOH, 3mL buffer, 50 mL oil
  
==Data==
+
Set 1:
  
==Notes==
+
Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C
 +
 
 +
*done for all samples
 +
 
 +
Set 2:
 +
 
 +
Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C
 +
 
 +
*only done for 0.25g sample
 +
 
 +
Set 3:
 +
 
 +
Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing
 +
 
 +
*done for all samples
 +
 
 +
Repeat a 3 Freeze-Thaw Cycles of 20 hours freezing and 4 hours thawing
 +
 
 +
==Observations==
 +
 
 +
For Set 1, our purpose in using the petri dishes was to create a larger surface area for the hydrogels to form. After preparing our water/oil emulsion and placing in the freezer for ~10min, it was apparent that although there would be a greater separation between the gels that form, there would still be significant adhesion between them. Because of this, we decided to only to do one sample in the same way using mineral oil, just to see if there was any difference. For Set 3, we used safflower oil and flash froze our sample prior to placing in the freezer. This method was done on all three samples.
  
  

Latest revision as of 21:28, 26 September 2017

BDLlogo notext lr.png Microgel Prep III Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


Objective

  • Use Safflower Oil (higher freezing point than mineral oil)
  • Freeze using Liquid Nitrogen before placing in freezer

Procedures

Make the following samples:

1) 0.5g PVOH, 4 mL buffer, 50 mL oil

2) 0.25g PVOH, 3 mL buffer, 50 mL oil

3) 0.14g PVOH, 3mL buffer, 50 mL oil

Set 1:

Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

  • done for all samples

Set 2:

Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

  • only done for 0.25g sample

Set 3:

Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing

  • done for all samples

Repeat a 3 Freeze-Thaw Cycles of 20 hours freezing and 4 hours thawing

Observations

For Set 1, our purpose in using the petri dishes was to create a larger surface area for the hydrogels to form. After preparing our water/oil emulsion and placing in the freezer for ~10min, it was apparent that although there would be a greater separation between the gels that form, there would still be significant adhesion between them. Because of this, we decided to only to do one sample in the same way using mineral oil, just to see if there was any difference. For Set 3, we used safflower oil and flash froze our sample prior to placing in the freezer. This method was done on all three samples.