Difference between revisions of "User:Allison K. Alix/Notebook/CHEM-581/2013/02/20"

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(Objective)
(Procedures)
Line 28: Line 28:
  
 
Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C
 
Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C
 +
 +
*done for all samples
  
 
Set 2:
 
Set 2:
Line 38: Line 40:
  
 
Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing
 
Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing
 +
 +
*done for all samples
 +
 +
Repeat a 3 Freeze-Thaw Cycles of 20 hours freezing and 4 hours thawing
  
 
==Data==
 
==Data==

Revision as of 08:52, 20 February 2013

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Objective

  • Use Safflower Oil (higher freezing point than mineral oil)
  • Freeze using Liquid Nitrogen before placing in freezer

Procedures

Make the following samples:

1) 0.5g PVOH, 4 mL buffer, 50 mL oil

2) 0.25g PVOH, 3 mL buffer, 50 mL oil

3) 0.14g PVOH, 3mL buffer, 50 mL oil

Set 1:

Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

  • done for all samples

Set 2:

Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

  • only done for 0.25g sample

Set 3:

Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing

  • done for all samples

Repeat a 3 Freeze-Thaw Cycles of 20 hours freezing and 4 hours thawing

Data

Notes