Difference between revisions of "User:Allison K. Alix/Notebook/CHEM-581/2013/02/20"

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Microgel Prep III</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Microgel Prep III</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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Latest revision as of 22:28, 26 September 2017

BDLlogo notext lr.png Microgel Prep III Report.pngMain project page
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  • Use Safflower Oil (higher freezing point than mineral oil)
  • Freeze using Liquid Nitrogen before placing in freezer


Make the following samples:

1) 0.5g PVOH, 4 mL buffer, 50 mL oil

2) 0.25g PVOH, 3 mL buffer, 50 mL oil

3) 0.14g PVOH, 3mL buffer, 50 mL oil

Set 1:

Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

  • done for all samples

Set 2:

Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

  • only done for 0.25g sample

Set 3:

Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing

  • done for all samples

Repeat a 3 Freeze-Thaw Cycles of 20 hours freezing and 4 hours thawing


For Set 1, our purpose in using the petri dishes was to create a larger surface area for the hydrogels to form. After preparing our water/oil emulsion and placing in the freezer for ~10min, it was apparent that although there would be a greater separation between the gels that form, there would still be significant adhesion between them. Because of this, we decided to only to do one sample in the same way using mineral oil, just to see if there was any difference. For Set 3, we used safflower oil and flash froze our sample prior to placing in the freezer. This method was done on all three samples.