User:Allison K. Alix/Notebook/CHEM-581/2013/02/15

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Objective

  • Vary the ratio of PVOH/Phosphate buffer to Mineral oil.

Description

We will look at the following solutions for preparing microgels:

1) 0.5g PVOH, 4 mL buffer, 50 mL oil

2) 0.25g PVOH, 3 mL buffer, 50 mL oil

3) 0.14g PVOH, 3mL buffer, 50 mL oil

In addition to this, we will cross link already formed hydrogels from 02/01/2013

Procedures

1) Heat ~75mL gluteraldehyde fixant solution (contains 0.1M HCl and Na2SO4) to 60°C

2) Place 5 hydrogels in solution with 1mL gluteraldehyde.

3) Gradually take 1 hydrogel out every 15 minutes and store in water.

4) Observe effects of crosslinkage

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.