User:Allison K. Alix/Notebook/CHEM-581/2013/02/11: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Preparing Microgels Part II</span> | ||
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==Objective== | ==Objective== | ||
* Prepare smaller hydrogels using a similar procedure to the one used on [[Allison K. Alix/Notebook/CHEM-581/2013/ | * Prepare smaller hydrogels using a similar procedure to the one used on [[User:Allison K. Alix/Notebook/CHEM-581/2013/02/06|2/06/13]] | ||
==Description== | ==Description== | ||
== | The nanogels prepared on 2/06/13 may be too small for our system. This being said, we are looking to devise another procedure using w/o emulsion that will make slightly larger hydrogels (~microscale) | ||
==Procedures== | |||
1) Add 0.5g of PVOH (MW = 89,000-98,000 g/mol) to 6mL phosphate buffer solution | |||
2) Heat at 100°C for ~15 minutes | |||
3) Pour into 50mL mineral oil and homogenize on the lower setting (rpm unknown- unlisted) for 1 min | |||
4) Place in freezer at -20°C for 20 hours. Thaw for 4 hours. Repeat this cycle for a total of 3 cycles. | |||
==Observations== | |||
-Size of hydrogels is still unknown, but we are hoping that using more PVOH/phosphate buffer solution and less mineral oil as well as homogenization at a lower speed will allow for the formation of larger hydrogels. | |||
[[Category:Course]] | [[Category:Course]] | ||
Revision as of 12:28, 18 February 2013
 Preparing Microgels Part II
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Objective
DescriptionThe nanogels prepared on 2/06/13 may be too small for our system. This being said, we are looking to devise another procedure using w/o emulsion that will make slightly larger hydrogels (~microscale) Procedures1) Add 0.5g of PVOH (MW = 89,000-98,000 g/mol) to 6mL phosphate buffer solution 2) Heat at 100°C for ~15 minutes 3) Pour into 50mL mineral oil and homogenize on the lower setting (rpm unknown- unlisted) for 1 min 4) Place in freezer at -20°C for 20 hours. Thaw for 4 hours. Repeat this cycle for a total of 3 cycles. Observations-Size of hydrogels is still unknown, but we are hoping that using more PVOH/phosphate buffer solution and less mineral oil as well as homogenization at a lower speed will allow for the formation of larger hydrogels.
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