Difference between revisions of "User:Allison K. Alix/Notebook/CHEM-581/2013/02/06"

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-Begin PVA microsphere preparation
 
-Begin PVA microsphere preparation
  
==Description==
+
==Procedure==
 +
 
 +
'''Preparation of 20mM phosphate buffer, pH = 7.4'''
 +
 
 +
[Base] = monosodium phosphate, 119.98 g/mol
 +
[Acid] = disodium phosphate, 141.96 g/mol
 +
 
 +
pH = pKa + log ([Base]/[Acid])
 +
 
 +
7.4 = 7.3 + log ([Base]/[Acid])
 +
 
 +
([Base]/[Acid]) = 1.2589
 +
 
 +
[Acid] + [Base] = 20mM
 +
 
 +
[Base] = 20mM - [Acid]
 +
 
 +
(20mM - [Acid])/[Acid] = 1.2589
 +
 
 +
[disodium phosphate] = 8.85mM
 +
 
 +
[Base] = 11.14mM
  
 
==Data==
 
==Data==

Revision as of 06:18, 6 February 2013

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Objective

-Begin PVA microsphere preparation

Procedure

Preparation of 20mM phosphate buffer, pH = 7.4

[Base] = monosodium phosphate, 119.98 g/mol [Acid] = disodium phosphate, 141.96 g/mol

pH = pKa + log ([Base]/[Acid])

7.4 = 7.3 + log ([Base]/[Acid])

([Base]/[Acid]) = 1.2589

[Acid] + [Base] = 20mM

[Base] = 20mM - [Acid]

(20mM - [Acid])/[Acid] = 1.2589

[disodium phosphate] = 8.85mM

[Base] = 11.14mM

Data

  • Add data and results here...

References

http://www.sciencedirect.com/science/article/pii/S0168365998000893


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