Difference between revisions of "User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/12"

From OpenWetWare
Jump to: navigation, search
(Procedure)
(Procedure)
 
(No difference)

Latest revision as of 14:38, 16 February 2014

BDLlogo notext lr.png Biomaterials Design Lab: Spring 2014 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • Take 1 mL aliquots of lysozyme-AuNP and BSA-AuNP synthesized yesterday.
  • Centrifuge aliquots and remove supernatant.
  • Retest all treatments with samples of pure fibers.

Procedure

  • 1ml aliquots were prepared for all newly synthesized AuNP's. Lysozyme: 35:1 55:1 110:1 ------- BSA: 100:1
  • All 1ml tubes for the 55:1 and 110:1 ratios were spun down in a centrifuge. Supernatant was removed in order to get pure fibers.
  • To the pure fiber samples and BSA AuNP's chelators, and buffers were added.
  • At this point we ran out of time.